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Effect of the percentage of GC content on the hybridization kinetics of the MB-target. Mean ± standard deviation from 6 independent microarrays measuring the mean fluorescence from at least 6 spots for <t>each</t> <t>microarray.</t> Three MBs with different stems were assayed consisting of 40 (blue), 60 (red) and 100 (fuchsia) % GC content in their stem. Arrow indicates the time of target application to the microarrays. Mean fluorescence was obtained from regions of interest (ROI) using the plot profile function from <t>ImageJ</t> for each microarray spot and measured overtime (see ). Then, means from all spots measured overtime were used to calculate the final mean ± standard deviation (see ).
Imagej Line Profile Tool, supplied by wavemetrics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Design of Hydrogel Silk-Based Microarrays and Molecular Beacons for Reagentless Point-of-Care Diagnostics"

Article Title: Design of Hydrogel Silk-Based Microarrays and Molecular Beacons for Reagentless Point-of-Care Diagnostics

Journal: Frontiers in Bioengineering and Biotechnology

doi: 10.3389/fbioe.2022.881679

Effect of the percentage of GC content on the hybridization kinetics of the MB-target. Mean ± standard deviation from 6 independent microarrays measuring the mean fluorescence from at least 6 spots for each microarray. Three MBs with different stems were assayed consisting of 40 (blue), 60 (red) and 100 (fuchsia) % GC content in their stem. Arrow indicates the time of target application to the microarrays. Mean fluorescence was obtained from regions of interest (ROI) using the plot profile function from ImageJ for each microarray spot and measured overtime (see ). Then, means from all spots measured overtime were used to calculate the final mean ± standard deviation (see ).
Figure Legend Snippet: Effect of the percentage of GC content on the hybridization kinetics of the MB-target. Mean ± standard deviation from 6 independent microarrays measuring the mean fluorescence from at least 6 spots for each microarray. Three MBs with different stems were assayed consisting of 40 (blue), 60 (red) and 100 (fuchsia) % GC content in their stem. Arrow indicates the time of target application to the microarrays. Mean fluorescence was obtained from regions of interest (ROI) using the plot profile function from ImageJ for each microarray spot and measured overtime (see ). Then, means from all spots measured overtime were used to calculate the final mean ± standard deviation (see ).

Techniques Used: Hybridization, Standard Deviation, Fluorescence, Microarray



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wavemetrics inc imagej line profile tool
Effect of the percentage of GC content on the hybridization kinetics of the MB-target. Mean ± standard deviation from 6 independent microarrays measuring the mean fluorescence from at least 6 spots for <t>each</t> <t>microarray.</t> Three MBs with different stems were assayed consisting of 40 (blue), 60 (red) and 100 (fuchsia) % GC content in their stem. Arrow indicates the time of target application to the microarrays. Mean fluorescence was obtained from regions of interest (ROI) using the plot profile function from <t>ImageJ</t> for each microarray spot and measured overtime (see ). Then, means from all spots measured overtime were used to calculate the final mean ± standard deviation (see ).
Imagej Line Profile Tool, supplied by wavemetrics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imagej+line+profile+tool/pmc09361048-96-7-15?v=wavemetrics+inc
Average 90 stars, based on 1 article reviews
imagej line profile tool - by Bioz Stars, 2026-07
90/100 stars
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Effect of the percentage of GC content on the hybridization kinetics of the MB-target. Mean ± standard deviation from 6 independent microarrays measuring the mean fluorescence from at least 6 spots for each microarray. Three MBs with different stems were assayed consisting of 40 (blue), 60 (red) and 100 (fuchsia) % GC content in their stem. Arrow indicates the time of target application to the microarrays. Mean fluorescence was obtained from regions of interest (ROI) using the plot profile function from ImageJ for each microarray spot and measured overtime (see ). Then, means from all spots measured overtime were used to calculate the final mean ± standard deviation (see ).

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Design of Hydrogel Silk-Based Microarrays and Molecular Beacons for Reagentless Point-of-Care Diagnostics

doi: 10.3389/fbioe.2022.881679

Figure Lengend Snippet: Effect of the percentage of GC content on the hybridization kinetics of the MB-target. Mean ± standard deviation from 6 independent microarrays measuring the mean fluorescence from at least 6 spots for each microarray. Three MBs with different stems were assayed consisting of 40 (blue), 60 (red) and 100 (fuchsia) % GC content in their stem. Arrow indicates the time of target application to the microarrays. Mean fluorescence was obtained from regions of interest (ROI) using the plot profile function from ImageJ for each microarray spot and measured overtime (see ). Then, means from all spots measured overtime were used to calculate the final mean ± standard deviation (see ).

Article Snippet: All microarray data were analyzed using the ImageJ line profile tool and Igor Pro v7 (WaveMetrics, Portland, OR, United States).

Techniques: Hybridization, Standard Deviation, Fluorescence, Microarray